Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance

Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance

Abstract

During the first meiotic division, crossovers (COs) between homologous chromosomes ensure their correct segregation. COs are produced by homologous recombination (HR)-mediated repair of programmed DNA double strand breaks (DSBs). As more DSBs are induced than COs, mechanisms are required to establish a regulated number of COs and to repair remaining intermediates as non-crossovers (NCOs). We show that the Caenorhabditis elegans RMI1 homolog-1 (RMH-1) functions during meiosis to promote both CO and NCO HR at appropriate chromosomal sites. RMH-1 accumulates at CO sites, dependent on known pro-CO factors, and acts to promote CO designation and enforce the CO outcome of HR-intermediate resolution. RMH-1 also localizes at NCO sites and functions in parallel with SMC-5 to antagonize excess HR-based connections between chromosomes. Moreover, RMH-1 also has a major role in channeling DSBs into an NCO HR outcome near the centers of chromosomes, thereby ensuring that COs form predominantly at off-center positions.

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Authors
  • von Haeseler, Arndt
  • Gallach, Miguel
  • Paulin Paz, Luis
  • Jantsch, Verena
  • Villeneuve, Anne M.
  • Tam, Angela
  • Habacher, Cornelia
  • Tang, Louis
  • Dello Stritto, Maria Rosaria
  • Mikl, Martin
  • Millonigg, Sophie
  • Woglar, Alexander
  • Hamminger, Patricia
  • Jagut, Marlène
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Shortfacts
Category
Journal Paper
Divisions
Bioinformatics and Computational Biology
Journal or Publication Title
PLoS Biology
ISSN
1544-9173
Publisher
PLOS Biology
Place of Publication
San Francisco, California
Number
3
Volume
14
Date
March 2016
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