NMD is required for timely cell fate transitions by fine-tuning gene expression and controlling translation

NMD is required for timely cell fate transitions by fine-tuning gene expression and controlling translation

Abstract

Cell fate transitions depend on balanced rewiring of transcription and translation programmes to mediate ordered developmental progression. Components of the nonsense-mediated mRNA decay (NMD) pathway have been implicated in regulating embryonic stem cell (ESC) differentiation, but the exact mechanism is unclear. Here we show that NMD controls the translation initiation factor Eif4a2 and its premature termination codon encoding isoform (Eif4a2PTC). NMD deficiency leads to translation of a specific truncated Eif4a2 protein, which elicits increased translation rates and causes significant delays in mouse ESC differentiation. Thereby a previously unknown feedback loop between NMD and translation initiation is established. Our results illustrate a clear hierarchy between KOs in severity of target deregulation and differentiation phenotype (Smg5 > Smg6 > Smg7), which highlights heterodimer-independent functions for Smg5 and Smg7. Together, our findings expose an intricate link between mRNA stability and translation initiation control that must be maintained for normal dynamics of cell state transitions.

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Authors
  • Galimberti, Elena
  • Sehlke, Robert
  • Huth, Michelle
  • Garmhausen, Michelle
  • Romeike, Merrit
  • Ramesmayer, Julia
  • Stummer, Sarah
  • Titz-Teixeira, Fabian
  • Herzog, Veronika A.
  • Chugunova, Anastasia
  • Leesch, Katrin Friederike
  • Holcik, Laurenz
  • Bücker, Christa
  • Pauli, Andrea
  • Schoefer, Christian
  • Ameres, Stefan L.
  • Smith, Austin
  • Beyer, Andreas
  • Leeb, Martin
  • Weipoltshammer5, Klara
  • von Haeseler, Arndt
  • Santini, Laura
  • Lackner, Andreas
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Shortfacts
Category
Journal Paper
Divisions
Bioinformatics and Computational Biology
Journal or Publication Title
bioRxiv Cold Spring Harbor Laboratory
ISSN
none
Publisher
bioRxiv
Date
7 July 2021
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